The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
Insigneo Institute for in silico Medicine, Sheffield, UK
Institute of Ophthalmology, Faculty of Brain Sciences, University College London, London, UK
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The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
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The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
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The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
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The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
Insigneo Institute for in silico Medicine, Sheffield, UK
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The Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, UK
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Insigneo Institute for in silico Medicine, Sheffield, UK
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, 9 , 10 ). This highlights the importance of studying the responses of multiple vascular in development and territories to experimental manipulations. Zebrafish embryos are a frequently used model to study vascular development and disease ( 11
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disrupting processes ( 22 , 32 , 33 ). In zebrafish embryos, vinculin associates with endothelial junctions that are remodelled by changes in blood flow ( 34 ) and endothelial expression of vinculin is important for angiogenesis in the postnatal mouse
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Heart and Vascular Center, Semmelweis University, Budapest, Hungary
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imaging. Although a powerful technique, the endogenous capability of smaller mammals to restore blood flow via post-ischaemic angiogenesis makes an assessment of therapeutic efficacy challenging ( 22 ). Zebrafish present themselves as an alternative model
Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal
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Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
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, 42 , 43 ). However, in zebrafish, VEGFA signaling was associated to the proliferation of angioblasts rather than to their migration. Instead, angioblasts rely on Elabela and Apelin activation of the Apelin receptor ( Aplnr ) signaling and on EC
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Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
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2014 102 312 – 320 . ( https://doi.org/10.1093/cvr/cvu057 ) 30 Major RJ Poss KD . Zebrafish heart regeneration as a model for cardiac tissue repair . Drug Discovery Today: Disease Models 2007 4 219 – 225 . ( https://doi.org/10.1016/j
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). Drosha, as well as Dicer, a key role in endothelial miRNA expression ( 7 ). Drosha-deficient zebrafish showed abnormalities in vascular development, and mice with an endothelial-specific knockout of Drosha exhibited disorganised, dilated vasculature and
Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands
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sexual dimorphisms may limit their translational value. Other animal models are also employed to study atherosclerosis, such as zebrafish. Compared to mice, zebrafish have an even smaller body size, develop more rapidly, and produce a large number of
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contributes to vessel maturation by the specification of the arterial vs the venous compartments. For example, in zebrafish and mice, endothelial progenitor cells express markers of both venous and arterial phenotype ( 4 , 5 ), and the exposure to blood flow
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phenotype is mainly regulated by flow ( 6 ). For instance, vascular remodeling in zebrafish has shown transformation of arterial intersegmental vessels into venous phenotypes to create efficient blood circulation ( 101 ). This phenomenon demonstrates the
ProCure Research Program, Instituto de Salud Carlos III, Madrid, Spain
OncoBell Program, Instituto de Salud Carlos III, Madrid, Spain
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ProCure Research Program, Instituto de Salud Carlos III, Madrid, Spain
OncoBell Program, Instituto de Salud Carlos III, Madrid, Spain
CIBERONC, Instituto de Salud Carlos III, Madrid, Spain
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( 11 ). Conversely, arteriogenesis requires the suppression of PI3K signalling ( 12 ). Using mouse and zebrafish models of sprouting angiogenesis, it was identified that PI3Kα regulates endothelial cell rearrangements and junctional remodelling within
