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Xiaojing Ma Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Hongfei Li Department of Biological Sciences, Mount Holyoke College, South Hadley, Massachusetts, USA

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Shuntian Zhu Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Zixuan Hong Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Weijing Kong Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Qihang Yuan Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Runlong Wu Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Zihang Pan Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Jing Zhang Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing, China

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Yahong Chen Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing, China

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Xi Wang Department of Biological and Environmental Engineering, Cornell University, Ithaca, New York, USA

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Kai Wang Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

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Introduction Organoids should comprise at least two cell types which further organize into a 3D structure recapitulating some of the key features of the native organ ( 1 ). Although organoids have gained significant attention in the academic

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Emily Warren Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA

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Sharon Gerecht Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA

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, high doses of TNFα suppressed angiogenesis. The presence of pericytes appeared to modulate this switch, where the presence of pericytes rescued the antiangiogenic effects of TNFα at high doses to become proangiogenic. Organoids Recently, there

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Jaana Schneider Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology, Vienna, Austria
Austrian Cluster for Tissue Regeneration, Vienna, Austria

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Marianne Pultar Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology, Vienna, Austria
Austrian Cluster for Tissue Regeneration, Vienna, Austria

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Wolfgang Holnthoner Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology, Vienna, Austria
Austrian Cluster for Tissue Regeneration, Vienna, Austria

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organoids/mini-organs, resulting in organ-on-a-chip systems, which rely on functional vasculature as well ( 4 ). As a rather new process 3D bioprinting has also been considered as an approach for successful vascularization with a wide range of applicability

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Eleonora Zucchelli National Heart and Lung Institute, Imperial College London, London, UK

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Qasim A Majid National Heart and Lung Institute, Imperial College London, London, UK

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Gabor Foldes National Heart and Lung Institute, Imperial College London, London, UK
Heart and Vascular Center, Semmelweis University, Budapest, Hungary

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cellular settings can provide us with consistent performance with reduced cell culture artefacts and permit continuous and quantitative imaging. We suggest that scaffold-based cell constructs, as well as organoids, spheroids, hydrogels, and bioprinting (3D

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Roberta Giordo Department of Medical Laboratory Sciences, College of Health Sciences, and Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates

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Panagiotis Paliogiannis Department of Medical, Surgical and Experimental Surgery, University of Sassari, Sassari, Italy

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Arduino Aleksander Mangoni Discipline of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Adelaide, Australia

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Gianfranco Pintus Department of Medical Laboratory Sciences, College of Health Sciences, and Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates
Department of Biomedical Sciences, University of Sassari, Viale San Pietro, Sassari, Italy

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as the lung and the kidney ( 20 ). In this regard, recent data obtained in engineered human blood vessel organoids derived frm induced pluripotent stem cells confirmed that SARS-CoV-2 can directly infect blood vessel cells. Indeed, SARS-CoV-2-infected

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Jamie I van der Vaart Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Robin van Eenige Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Patrick C N Rensen Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Sander Kooijman Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands

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model heart diseases in vitro , including the use of human organoids (i.e. heart-on-a-chip), and 3D-cell printing techniques ( 114 , 115 ). These in vitro techniques are ideal for drug testing and determining therapies in personalized medicine

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Makeda Stephenson Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland, USA

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Daniel H Reich Department of Physics and Astronomy, The Johns Hopkins University, Baltimore, Maryland, USA

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Kenneth R Boheler Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland, USA

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of tissues from human pluripotent stem cells . Current Stem Cell Reports 2016 43 – 51 . ( https://doi.org/10.1007/s40778-016-0030-z ) 27330934 87 Clevers H . Modeling development and disease with organoids . Cell 2016 1586 – 1597

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