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OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms

Majid Almansouri

Majid Almansouri Department of Infection Immunity and Cardiovascular Disease, University of Sheffield, Medical School, Sheffield, UK

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Pooja Patel

Pooja Patel Department of Infection Immunity and Cardiovascular Disease, University of Sheffield, Medical School, Sheffield, UK

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Janet Chamberlain

Janet Chamberlain Department of Infection Immunity and Cardiovascular Disease, University of Sheffield, Medical School, Sheffield, UK

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Sheila Francis

Sheila Francis Department of Infection Immunity and Cardiovascular Disease, University of Sheffield, Medical School, Sheffield, UK
Healthy Lifespan Institute HELSI, University of Sheffield, Medical School, Sheffield, UK

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as control. In some experiments, cells were pre-incubated with NLRP3 inflammasome inhibitor (MCC950; 10 μM), caspase-1 inhibitor I (YVAD; 50 μM) or gasdermin D inhibitor (disulfiram; 0.5–1.0 μM) for 1 h before the addition of oxLDL. Cells were