- Approval – Ensure all authors have seen and approved the final version of the article prior to submission and are aware it is being submitted to Vascular Biology.
- Open Access – The appropriate Open Access licence must be selected on submission. Authors are responsible for ensuring any funder mandates are followed. For further details, please see the Open Access policy.
- Charges – Bioscientifica is sponsoring the Article Publication Charge during the launch years; as such authors can publish completely free of charge. Further details are available on our publication charges page.
- Ethical compliance – All articles are required to meet the requirements outlined in our ethical policy. Ensure you have included all relevant ethical approval statements.
- Author list – All authors must be listed on the title page and entered on the ScholarOne Manuscripts submission in the correct order. Ensure all author email addresses provided are valid. Author information entered into ScholarOne Manuscripts will be used to generate PubMed listings for published papers.
- Cover letter – This letter should introduce your paper and outline why your work is important and suitable for publication at this time.
- English language – Non-native English speakers are encouraged to have their manuscript professionally edited before submission. See Bioscientifica’s recommended English language editing services. Manuscripts may be written in either UK or US English.
- Please be aware that the combined size of your files should not exceed 40 MB;
- For article text: TXT, DOC, DOCX, TEX. We are unable to accept PDF files for article text for revised manuscripts, but can do so for first submissions;
- For figures: EPS, TIFF, JPG.
Article types; Original Research
1. Title Page
- Title (maximum 85 characters)
- All authors' names and full addresses
- Corresponding author’s postal and email address
- A short title (maximum 46 characters, including spaces)
- A minimum of four keywords describing the manuscript
- Word count of the full article, excluding references and figure legends
The abstract should be a single paragraph of not more than 250 words, clearly stating the objective of the study or review, the methods used (where applicable), and summarizing results and conclusions. Avoid abbreviations and references in this section.
The introduction should set the study in context by briefly reviewing relevant knowledge of the subject; follow this with a concise statement of the hypothesis and objectives of the study. The introduction should rarely exceed 3 pages of double-spaced text.
4. Materials and methods
Provide sufficient information for other workers to repeat the study. If well-established methods are used give a reference to the technique and provide full details of any modifications.
- Include the source of chemicals, reagents and hormones and give the manufacturer’s name in parentheses.
- Give the generic name, dose and route of administration for drugs.
- Specify the composition of buffers, solutions and culture media.
- Use SI symbols, give concentrations in mol/L and define the term % as w/v or v/v for all solutions. For international units use IU (U should be used for enzyme activity).
- Specify the type of equipment (microscopes/objective lenses, cameras, detectors) used to obtain images.
- Specify any image acquisition software used, and give a description of specialized techniques requiring large amounts of processing, such as confocal, deconvolution, 3D reconstructions, or surface and volume rendering.
7. Declaration of interest, Funding, Contributions and Acknowledgements
Please either (a) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported; or (b) fully declare any financial or other potential conflict of interest.
- Employment and consultancies
- Grants, fees and honoraria
- Ownership of stock or shares
- Patents (pending and actual)
- Board membership
Please detail all of the sources of funding relevant to the research reported in the following format:
This work was supported by the Medical Research Council (grant numbers xxxx, yyyy); the Wellcome Trust (grant number xxxx); and Tommy’s Baby charity (grant number xxxx).
Where research has not been funded please state the following:
This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
Author contribution statement (compulsory)
Please include a statement concisely specifying the contribution of each co-author. Use author initials to indicate contributions, for example: CP conceived the study and wrote the paper. GF performed experiments and analysed datacontribution of each co-author.
Please be as brief as possible.
Any unpublished work (personal communications, manuscripts in preparation and manuscripts submitted but not yet accepted for publication) must be referred to in the text and not listed in the references.
Give the full list of authors, including their initials. For example:
(A Stone, J Brown & M R Smith, unpublished observations)
(J Brown, personal communication)
Articles accepted for publication but not yet published may be listed as ‘in press’ in the reference list, using the current year as the publication year. If an ‘in press’ article is included in the Accepted Manuscript service or a similar scheme, then the Digital Object Identifier (DOI) should be included; otherwise, provide a copy of the article as a supplementary file for reviewing purposes.
In the text
Cite references in the text in numerical order.
In the reference list
List references in the order they are cited in the text.
Reference in the following format:
1. Inkster S, Yue W & Brodie A. Human testicular aromatase: immunocytochemical and biochemical studies. Journal of Clinical Endocrinology and Metabolism 1995 80 1941–1947.
2. Matthews CH, Borgato S, Beck-Peccoz P, Adams M, Tone Y, Gambino G, Casagrande S, Tedeschini G, Benedetti A & Chatterjee VK. Primary amenorrhea and infertility due to a mutation in the β-subunit of follicle-stimulating hormone. Nature Genetics 1993 5 83–86.
3. Royer P. Hormonal regulation of calcium metabolism: biology and pathology. In Pediatric Endocrinology, edn 2, ch 6, pp 477–543. Eds J-C Job & M Pierson. New York: John Wiley & Sons, 1981.
Please use Vancouver style.
- Number tables in the order they are cited in the text
- Include a title – a single sentence at the head of the table that includes the name of the organism studied
- Use footnotes to provide any additional explanatory material, cross-referenced to the column entries
- Give a short heading for each column
- Do not use internal horizontal or vertical lines, colour or shading
- Explain all abbreviations used in the table in the footnotes
- Number figures in the order they are cited in the text
- Include legends to all figures, giving the figure number, keys to any symbols used, the name of the organism studied, the names of any statistical tests used and the probability levels used for comparisons
- Label figure sections as A, B etc in the top left-hand corner
- Use Arial or a similar sans-serif font for text labels
- Do not enclose figures in boxes
- Indicate magnification by a scale bar in the bottom right-hand corner of the image and give the measurement in the legend
- Use the preferred symbols of closed and open circles, squares and triangles. Ensure that symbols are large enough to be read clearly when the figure is reduced for publication
- Use Courier or a similar non-proportional font for amino acid, DNA, RNA and PCR primer sequences and highlight sections of homology between sequences with grey shading
File types and resolution
Vascular Biology is committed to publishing high quality figures. EPS or TIFF files are preferred and files should be exported in Illustrator-compatible format. Avoid using PowerPoint or Word files for figures.
- Line images/graphs: EPS, TIFF, high-resolution PDF, AI (Adobe Illustrator); resolution at final published size: 1200 dpi
- Half-tone (greyscale) images: TIFF, high-resolution PDF, JPG; resolution at final published size: 600 dpi
- Colour images: TIFF, high-resolution PDF, JPEG. EPS or AI files can be used for graphical data and illustrations that don’t include photographs; resolution at final published size: 300 dpi; RGB format for colour.
Article Types: Reviews
- Title page
- Declaration of interest, Funding, Author contributions statements (where appropriate)
- Figure legends
Article Types: Mini-Reviews
Article Types: Editorials
(Supplementary Table 1)
(Supplementary Figures 1 and 2)
Human subjects research
- Give the full binomial Latin names for all experimental animals other than common laboratory animals
- State the breed or strain and source of animals, and give details of age, weight, sex and housing
- Detail the procedures and anaesthetics used, including doses given.
Experiments with genetically engineered mice
We require that all cell lines are authenticated for correct origin. Specifically, the author should include the following information supporting the authentication of lines:
- Source of cell lines. Gifts of cell lines from individuals will not be acceptable
- Please state what the method of authentication is. For example, ATCC uses STRS analysis
- State the passage number(s) of cell lines used for the experiments described in the submission. Unless the research is specifically about senescence, lines >35 passages would not be acceptable.
- In gene and protein symbols, substitute Greek letters with the corresponding roman letter, eg TGFBR2 not TGFβR2
- Avoid hyphens unless they are part of the approved symbol, eg IGF1 not IGF-1
- Use arabic rather than roman numerals, eg BMPR2 not BMPRII
Mice and rats
- Gene symbols should be in italics with only the first letter capitalised, eg Sox2
- Protein designations should be the same as the gene symbols except that all letters should be capitalised and in roman (ie not italicised), eg SOX2
- Use symbols approved by the International Committee on Standardized Genetic Nomenclature for Mice and the Rat Genome and Nomenclature Committee, which can be queried at the MGI website
Humans, non-human primates and domestic species
- Gene symbols should be in italics with all letters capitalised, eg SOX2
- Protein designations should be the same as the gene symbols but not italicised, eg SOX2
- Use symbols approved by the HUGO Gene Nomenclature Committee (HGNC)
- Gene symbols should be in italics with all letters in lower case, eg sox2
- Protein designations should be the same as the gene symbols but not italicised and with the first letter capitalised, eg Sox2
- Use symbols approved by the Zebrafish Nomenclature Committee (ZNC), which can be queried at the ZFIN website
Digital image integrity
Burry RW 2011 Controls for immunocytochemistry: An update. Journal of Histochemistry & Cytochemistry 59 6–12. (doi:10.1369/jhc.2010.956920)
Saper CB 2009 A guide to the perplexed on the specificity of antibodies. Journal of Histochemistry & Cytochemistry 57 1–5. (doi:10.1369/jhc.2008.952770)
When several t-tests are employed, authors should be aware that nominal probability levels no longer apply. Accordingly, the multiple t-test, multiple range test, or similar techniques to permit simultaneous comparisons should be employed. Also, in lieu of using several t-tests, it is often more appropriate to utilize an analysis of variance (ANOVA) to permit pooling of data, increase the number of degrees of freedom, and improve reliability of results. Authors should use appropriate nonparametric tests when the data depart substantially from a normal distribution.
In presenting results of linear regression analyses, it is desirable to show 95% confidence limits.
- Describe the numbers of experimental units used and the way in which they have been allocated to treatments
- Justify the omission of any observations from the analysis
- Describe methods of analysis precisely and state any necessary assumptions, as these may affect the conclusions that can be drawn from the experiment
A preprint is a version of the article prior to submission to the journal for peer review, and has not been copyedited or typeset.
Bioscientifica allows deposition of preprints to recognized repositories, such as bioRxiv, provided that Bioscientifica is informed of this at the time of submission and it does not infringe any subsequent copyright or licence agreement.
Upon final publication, authors are required to add a link from the preprint to the published article (version of record).
Depositing data in public databases
Authors are strongly encouraged to deposit data sets in appropriate public databases, such as GenBank or Gene Expression Omnibus (GEO). Authors should include the relevant database identifiers and accession numbers for deposited sequences within the manuscript using the following format: Database: xxxx, e.g: GEO: GSE6364. Authors are also required to provide the URL for the sequence(s).
Please contact the editorial office if you have a query about relevant databases.
Licence and Copyright
Authors retain copyright of their articles and may choose to publish under the terms of the Creative Commons Attribution 4.0 International (CC BY); Attribution-NonCommercial 4.0 International (CC BY-NC); or Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND) licences. Please see Gold open access licenses for further information. Authors are permitted to copy and redistribute their work within the terms of their selected license. In the latter two cases authors also grant Bioscientifica Ltd commercial rights.
Authors who feel they have grounds to appeal a rejection decision should send a rebuttal letter to the editorial office, detailing the reasons for the appeal. Rebuttals will be considered by the Editor-in-Chief, often in consultation with the Editorial Board Member who handled the paper. Decisions on appeals are final.